Acetate salt
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| CLK-110-S | 0,5 mg (1 μmol) | 146,36 | Add to Basket/Quote Add to Notepad |
| CLK-110-L | 10 x 0,5 mg (10 μmol) | 730,25 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C22H28N10O4 (free amine)
Molecular Weight: 496.53 g/mol (free amine)
Exact Mass: 496.23 g/mol (free amine)
Purity: ≥ 95 % (HPLC)
Form: solid
Color: colorless
Solubility: DMSO, PBS (up to 50 mM tested) pH adjusted to 5.0
Spectroscopic Properties: λ 268 nm, ε 19.5 L mmol-1 cm-1 (0.1 M HCl)
Description:
Gee et al.[1] reported a non-radioactive alternative to analyze newly synthesized proteins in cell culture that is based on 5'-Azido-puromycin, an azide analog of puromycin.
5'-Azido-puromycin is cell-permeable and incorporates into the C-terminus of translating polypeptide chains thereby stopping translation.
The resulting truncated C-terminal azide-labeled proteins can subsequently be detected via copper-free click chemistry that offers the choice to introduce a Biotin group (via DBCO-modified Biotin) for subsequent purification tasks or a fluorescent group (via DBCO-modified fluorescent dyes) for subsequent microscopic imaging.
BIOZ Product Citations:
Selected References:
Gee et al. (2016) Puromycin Analogues Capable of Multiplexed Imaging and Profiling of Protein Synthesis and Dynamics in Live Cells and Neurons. Angew. Chem. Int.Ed.55: 4933.
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