We extended our portfolio of Fluorescein-labeled nucleotides for non-radioactive labeling of nucleic acids (Tab. 1).
Fluorescein has been a very popular choice for many years due to its brightness, stability and good water solubility. Fluorescein-nucleotide conjugates are efficiently incorporated into DNA and RNA as substitute for their natural counterparts. The excitation maximum of the fluorophore is well matched to the argon ion laser (488 nm) and despite the development of alternatives with increased photostability, it is still the gold standard in a broad range of fluorescence applications.
| Emission color |
Nucleotide | Cat. No. | Application | Specification |
|---|---|---|---|---|
| NTP | ||||
| green | Fluorescein-12-UTP | NU-821-FAMX | RNA Labeling | Purity: >/= 95 % (HPLC) Concentration: 5.0 mM – 5.5 mM pH: 7.5 ±0.5 |
| Fluorescein-12-CTP | NU-831-FAMX | RNA Labeling | Purity: >/= 95 % (HPLC) Concentration: 5.0 mM – 5.5 mM pH: 7.5 ±0.5 |
|
| dNTP | ||||
| green | Fluorescein-12-dUTP | NU-803-FAMX | DNA Labeling | Purity: >/= 95 % (HPLC) Concentration: 1.0 mM – 1.1 mM pH: 7.5 ±0.5 |
| Fluorescein-12-dCTP | NU-809-FAMX | DNA Labeling | Purity: >/= 95 % (HPLC) Concentration: 1.0 mM – 1.1 mM pH: 7.5 ±0.5 |
|
| Fluorescein-12-dATP | NU-1611-FAMX | DNA Labeling | Purity: >/= 95 % (HPLC) Concentration: 1.0 mM – 1.1 mM pH: 7.5 ±0.5 |
|
| Fluorescein-12-dGTP | NU-1615-FAMX | DNA Labeling | Purity: >/= 95 % (HPLC) Concentration: 1.0 mM – 1.1 mM pH: 7.5 ±0.5 |
|
| ddNTP | ||||
| green | Fluorescein-12-ddUTP | NU-1619-FAMX | DNA Labeling | Purity: >/= 95 % (HPLC) Concentration: 1.0 mM – 1.1 mM pH: 7.5 ±0.5 |
| Fluorescein-12-ddCTP | NU-850-FAMX | DNA Labeling | Purity: >/= 95 % (HPLC) Concentration: 1.0 mM – 1.1 mM pH: 7.5 ±0.5 |
|
| Fluorescein-12-ddATP | NU-1612-FAMX | DNA Labeling | Purity: >/= 95 % (HPLC) Concentration: 1.0 mM – 1.1 mM pH: 7.5 ±0.5 |
|
Please note: In addition to single nucleotides, we also offer (d)UTP-based DNA (Cat. No. APP-101-FAMX) and RNA (Cat. No. RNT-101-FAMX) Labeling Kits for the preparation of Fluorescein-modified probes.
Contact us if you are interested in different Fluorescein-labeled nucleotides!
