Now available: High concentration labeled nucleotides for efficient DNA and RNA Labeling

Jena Bioscience now offers 10 mM solutions of Cy3-, Cy5- and 5 mM solutions of Biotin- labeled nucleotides for efficient enzymatic, non-radioactive labeling of RNA (Tab. 1) and DNA (Tab. 2).

Cy3, Cy5 and Biotin are among the most used labels for the generation of non-radioactive DNA and RNA probes due to their excellent enzymatic incorporation properties and detection options. Read more in our brochure below:

Non-radioactive Labeling of DNA and RNA

Table 1: Enzymatic incorporation of fluorescent nucleotides for RNA Labeling

yPAP: Yeast Poly A Polymerase; TdT: Terminal deoxynucleotidyl Transferase.

cRNA Labeling
by in vitro Transcription with... 3'-RNA Labeling by…

Emission
color Nucleotide Cat. No. T7 RNA Polymerase T3 RNA Polymerase SP6 RNA Polymerase yPAP TdT T4 RNA Ligase

UTP

yellow UTP-Cy3 - high concentration NU-821-CY3-HC ✔¹ n/a n/a n/a n/a -

red UTP-Cy5 - high concentration NU-821-CY5-HC ✔¹ n/a n/a n/a n/a -

CTP

yellow CTP-Cy3 - high concentration NU-831-CY3-HC ✔¹ n/a n/a n/a n/a -

red CTP-Cy5 - high concentration NU-831-CY5-HC ✔¹ n/a n/a n/a n/a -

¹: in-house tested
n/a: no data available
- : not applicable

Table 2: Enzymatic incorporation of biotinylated nucleotides for DNA Labeling

PCR: Polymerase chain reaction with Taq Polymerase; NT: Nick Translation with DNAse I / DNA Polymerase I; Primer Extension: Primer Extension with Klenow 3'-5'exo-; 3'-End Labeling: Incorporation with Terminal deoxynucleotidyl Transferase (TdT); RT: Reverse Transcription with Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT).

DNA Labeling cDNA Labeling

Nucleotide Cat. No. PCR NT Primer Extension 3' End Labeling RT

Biotin-11-dATP - high concentration NU-1175-BIOX-HC ✔¹ n/a n/a n/a n/a

Biotin-11-dCTP - high concentration NU-809-BIOX-HC ✔¹ n/a n/a n/a n/a

¹: in-house tested
n/a: no data available
- : not applicable

Please note: In addition to the brand new 5 mM Biotin-labeled dNTP solutions, we also offer High Concentration Biotin-11-UTP (NU-821-BIOX-HC) as a 75 mM solution for RNA-labeling.

Questions or inquiries?

The labeled nucleotide analog you are interested in is not available as a high concentration solution? Just contact us for more information or an individual quotation!

Now available: High concentration labeled nucleotides for efficient DNA and RNA Labeling

Table 1: Enzymatic incorporation of fluorescent nucleotides for RNA Labeling

Table 2: Enzymatic incorporation of biotinylated nucleotides for DNA Labeling

Questions or inquiries?

©2001 – 2024 Jena Bioscience GmbH

			cRNA Labeling by in vitro Transcription with...			3'-RNA Labeling by…
Emission color	Nucleotide	Cat. No.	T7 RNA Polymerase	T3 RNA Polymerase	SP6 RNA Polymerase	yPAP	TdT	T4 RNA Ligase
UTP
yellow	UTP-Cy3 - high concentration	NU-821-CY3-HC	✔¹	n/a	n/a	n/a	n/a	-
red	UTP-Cy5 - high concentration	NU-821-CY5-HC	✔¹	n/a	n/a	n/a	n/a	-
CTP
yellow	CTP-Cy3 - high concentration	NU-831-CY3-HC	✔¹	n/a	n/a	n/a	n/a	-
red	CTP-Cy5 - high concentration	NU-831-CY5-HC	✔¹	n/a	n/a	n/a	n/a	-

		DNA Labeling				cDNA Labeling
Nucleotide	Cat. No.	PCR	NT	Primer Extension	3' End Labeling	RT
Biotin-11-dATP - high concentration	NU-1175-BIOX-HC	✔¹	n/a	n/a	n/a	n/a
Biotin-11-dCTP - high concentration	NU-809-BIOX-HC	✔¹	n/a	n/a	n/a	n/a